Journal: Journal of Neuroinflammation

Article Title: Chemerin-9 is neuroprotective in APP/PS1 transgenic mice by inhibiting NLRP3 inflammasome and promoting microglial clearance of Aβ

doi: 10.1186/s12974-024-03325-y

Figure Lengend Snippet: Disease-associated microglial marker is regulated by Chemerin-9. A Numbers of genes that were upregulated or downregulated and the volcano plot of DEGs between the chemerin-9-treated APP/PS1 mice versus the vehicle-treated APP/PS1 mice. B Bubble chart showing significant enrichment of DEGs in various pathways. C GSEA of protein digestion and absorption pathway of chemerin-9-treated APP/PS1 mice versus vehicle-treated APP/PS1 mice. D GSEA of the NOD-like receptor signaling pathway of chemerin-9-treated APP/PS1 mice versus vehicle-treated APP/PS1 mice. E Immunofluorescent staining of Iba1, Clec7a, and 6E10 in the hippocampus. F Quantification of Iba1-positive cell number within 15 μm radius distance around the Aβ plaque ( n = 3). G Percentage of Clec7a and Iba1-positive area versus Aβ plaque area ( n = 3). H Representative immunoblotting bands of APP, BACE1, PS1, PEN2, and Nicastrin in the hippocampus. I Quantitative analysis of APP/actin, BACE1/actin, PS1/actin, PEN2/actin, and Nicastrin/actin ( n = 3). * P < 0.05. PS1 = Presenilin 1; C9-H = High dose of chemerin-9; C9-L = Low dose of chemerin-9

Article Snippet: After blocking with 5% BSA in TBS-T, the membranes were subjected to overnight incubation at 4 °C with the primary antibodies listed below: synaptophysin (SYN) (A19122, 1:1000, Abclonal, Wuhan, China), postsynaptic density protein-95 (PSD95) (A6194, 1:1000, Abclonal, Wuhan, China), nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) (GB114320, 1:1000, Servicebio, Wuhan, China), apoptosis-associated speck-like protein containing a CARD (ASC) (10500-1-AP, 1:1000, Proteintech, IL, USA), caspase1 (A0964, 1:1000, Abclonal, Wuhan, China), APP (GB112075, 1:1000, Servicebio, Wuhan, China), Presenilin 1(PS1) (GB11779, 1:1000, Servicebio, Wuhan, China), PEN2 (A8678, 1:1000, Abclonal, Wuhan, China), BACE1 (A11533, 1:1000, Abclonal, Wuhan, China), Nicastrin (A0128, 1:1000, Abclonal, Wuhan, China), 6E10 (803001, 1:200, Biolegend, San Diego, USA), and β-actin (20536-1-AP, 1:1000, Proteintech, IL, USA).

Techniques: Marker, Staining, Western Blot